![]() ![]() Here, we employed the as isobaric tags for relative and absolute quantification (iTRAQ) technique to investigate the effects of S. exigua through comparative proteomic analysis can identify the key proteins expressed in response to the microbial infection. Understanding the immune defense mechanism of S. ̅ detoxification activity, catalyzed by the SOR–ferrocyanide complex, which does not conduct to the production of the toxic H2O2 species.Įntomopathogenic bacteria Serratia marcescens is widely used as an environmentally friendly biocontrol agent against various pests, including Spodoptera exigua.Altogether, these data describe an unprecedented O2 Accordingly, in vivoexperiments showed that formation of the SOR–ferrocyanide complex increased the antioxidant capabilities of SOR expressed in an Escherichia coli sodA sodB recA mutant strain. Surprisingly, the toxic H2O2 species is no longer the reaction product. A one-electron redox chemistry appears to be carried out by the ferrocyanide moiety of the complex, whereas the SOR iron site remains in the reduced state. However, the presence of the ferrocyanide adduct markedly modifies the reaction mechanism of SOR, with the formation of transient intermediates different from those observed for SOR alone. The complex reacts very efficiently with O2 ̅ by pulse and γ-ray radiolysis, infrared, and UV-visible spectroscopies.Here, we have investigated in detail the reactivity of the SOR–ferrocyanide complex with O2 Recently, the SOR active site, a ferrous iron in a pentacoordination, was shown to have the ability to form a complex with the organometallic compound ferrocyanide. ̅ detoxifying enzymes have been described so far, superoxide dismutase and superoxide reductase (SOR), both forming H2O2 as a reaction product.̅ is a toxic by-product of oxygen metabolism.Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Sperm membrane behaviour during cooling and cryopreservation. Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction. In: Henkel R., Samanta L., Agarwal A., editors. Role of oxidative stress in the etiology of male infertility and the potential therapeutic value of antioxidants. Advances in stallion semen cryopreservation. ![]() We can thus conclude that, in stallions, the specific activity of SOD in the seminal plasma of a given ejaculate might be related to its freezability.Īntioxidant enzymes cryopreservation equine seminal plasma sperm.Īlvarenga M.A., Papa F.O., Neto C.R. We observed that, while the specific activities of CAT, GPX, and GSR were similar between GFE and PFE, that of SOD was significantly ( p < 0.05) higher in GFE than in PFE. Sperm motility and viability were evaluated before and after cryopreservation, and ejaculates were classified as of good (GFE) or poor freezability (PFE) based on total motile and viable spermatozoa at post-thaw. The second aliquot was extended and then processed for cryopreservation. The first one was used to evaluate the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) in seminal plasma. With this purpose, 16 ejaculates were collected from 14 healthy stallions, and each ejaculate was split into two aliquots. Against this background, this study was designed to investigate whether the activities of the main stallion seminal plasma antioxidant enzymes are related to sperm cryotolerance. ![]() Therefore, stallion seminal plasma components may have an impact on ejaculate freezability. While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains the main source of antioxidant enzymes able to scavenge these reactive oxygen species. ![]()
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